Raised in the Chicago area, Seana Kofoed attended Northwestern University as well as The Royal National Theatre in London. She began her stage career in Chicago, appearing in productions at The Goodman Theatre, The Court Theatre and Victory Gardens Theatre before moving to New York. In New York, Kofoed was best known for productions on and off Broadway. Her Broadway credits include "Proof" with Jennifer Jason Leigh and "Night Must Fall" with Matthew Broderick. Off-Broadway credits include several productions at Manhattan Theatre Club, the Atlantic Theatre Company, and MCC. She received a Drama Desk Nomination for Best Supporting Actress in Shelagh Stephenson's "An Experiment with an Air Pump". Since moving to Los Angeles, Kofoed has recurred and guested on countless television shows, and was a series regular on Jenny Bicks' Men in Trees, and on the Jamie Denbo/Jenji Kohan comedy, American Princess. Also a writer, Kofoed wrote and produced the feature film, 30 Miles from Nowhere, a ReFrame stamp recipient for its diverse and gender-balanced cast and crew. She wrote the short film, Together Apart, in which she starred with her husband, Jason Antoon, and she wrote and starred in Donna's Revenge: Confessions of an Ex-Contestant, a comedy about the aftermath of reality television fame.